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Vacuum-assisted cell loading enables shear-free mammalian microfluidic culture

Microfluidic perfusion cultures for mammalian cells provide a novel means for probing single-cell behavior but require the management of culture parameters such as flow-induced shear stress. Methods to eliminate shear stress generally focus on capturing cells in regions with high resistance to fluid...

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Detalhes bibliográficos
Main Authors: Kolnik, Martin, Tsimring, Lev S, Hasty, Je
Formato: Artigo
Idioma:Inglês
Publicado em: 2012
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3510264/
https://ncbi.nlm.nih.gov/pubmed/22961584
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c2lc40569e
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