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Inorganic pyrophosphatase crystals from Thermococcus thioreducens for X-ray and neutron diffraction

Inorganic pyrophosphatase (IPPase) from the archaeon Thermococcus thioreducens was cloned, overexpressed in Escherichia coli, purified and crystallized in restricted geometry, resulting in large crystal volumes exceeding 5 mm(3). IPPase is thermally stable and is able to resist denaturation at tempe...

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Detalhes bibliográficos
Main Authors: Hughes, Ronny C., Coates, Leighton, Blakeley, Matthew P., Tomanicek, Steve J., Langan, Paul, Kovalevsky, Andrey Y., García-Ruiz, Juan M., Ng, Joseph D.
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3509969/
https://ncbi.nlm.nih.gov/pubmed/23192028
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309112032447
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