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Cell-free coupled transcription-translation system for investigation of linear DNA segments.

Heretofore the DNA-directed coupled transcription-translation system, most useful in gene expression analysis, has been limited to the use of circular or long linear DNAs. Linear DNAs are degraded in this system by an exonucleolytic activity that can be eliminated by making the synthetic extracts fr...

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Autors principals: Yang, H L, Ivashkiv, L, Chen, H Z, Zubay, G, Cashel, M
Format: Artigo
Idioma:Inglês
Publicat: 1980
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC350434/
https://ncbi.nlm.nih.gov/pubmed/6261235
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