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Plasmid cDNA-directed protein synthesis in a coupled eukaryotic in vitro transcription-translation system.
A system is described in which transcription of cDNA clones by bacteriophage T7 RNA polymerase is coupled to translation in the micrococcal nuclease treated rabbit reticulocyte lysate in a single reaction of coupled transcription-translation. The monovalent and divalent cation requirements for trans...
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| Vydáno v: | Nucleic Acids Res |
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| Hlavní autoři: | , , , , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
Oxford University Press
1992
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| On-line přístup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC334274/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1383935/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/20.19.4987 |
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