Exchange kinetics of the Schiff base proton in bacteriorhodopsin.
Using rapid mixing techniques and resonance Raman spectroscopy, we have found that the 1H/2H exchange time for the Schiff base proton of bacteriorhodopsin in purple membrane is 4.7 msec, when experiments are carried out at pH 2 or pH 7 at room temperature in the dark. We argue that diffusion of neut...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1980
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC350327/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6256745/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.77.11.6571 |
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