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Exchange kinetics of the Schiff base proton in bacteriorhodopsin.

Using rapid mixing techniques and resonance Raman spectroscopy, we have found that the 1H/2H exchange time for the Schiff base proton of bacteriorhodopsin in purple membrane is 4.7 msec, when experiments are carried out at pH 2 or pH 7 at room temperature in the dark. We argue that diffusion of neut...

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Detalles Bibliográficos
Publicado en:Proc Natl Acad Sci U S A
Autores principales: Ehrenberg, B, Lewis, A, Porta, T K, Nagle, J F, Stoeckenius, W
Formato: Artigo
Lenguaje:Inglês
Publicado: National Academy of Sciences 1980
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Acceso en línea:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC350327/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6256745/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.77.11.6571
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