Permeabilization of animal cells for kinetic studies of intracellular enzymes: in situ behavior of the glycolytic enzymes of erythrocytes.

Intracellular enzymes in erythrocytes can be made accessible for in situ kinetic studies by treating the cells with bifunctional reagents to crosslink proteins, thus creating a network that allows subsequent permeabilization by delipidation without escape of intracellular proteins. Dimethyl suberimi...

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Vydáno v:	Proc Natl Acad Sci U S A
Hlavní autoři:	Aragón, J J, Felíu, J E, Frenkel, R A, Sols, A
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	National Academy of Sciences 1980
Témata:	Research Article
On-line přístup:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC350276/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6450416/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.77.11.6324
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