Permeabilization of animal cells for kinetic studies of intracellular enzymes: in situ behavior of the glycolytic enzymes of erythrocytes.
Intracellular enzymes in erythrocytes can be made accessible for in situ kinetic studies by treating the cells with bifunctional reagents to crosslink proteins, thus creating a network that allows subsequent permeabilization by delipidation without escape of intracellular proteins. Dimethyl suberimi...
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| Pubblicato in: | Proc Natl Acad Sci U S A |
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| Autori principali: | , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
National Academy of Sciences
1980
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC350276/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6450416/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.77.11.6324 |
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