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Growth of T-lymphoma cells in serum-free medium: lack of involvement of the cyclic AMP pathway in long-term cultures.

We have developed a serum-free, chemically defined growth medium containing casein, insulin, transferrin, testosterone, and linoleic acid in Dulbecco's modified Eagle's medium/Ham's F12 medium, 1:1 (vol/vol), for growing murine T lymphomas. This medium supports the growth in suspensio...

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Bibliographic Details
Main Authors: Darfler, F J, Murakami, H, Insel, P A
Format: Artigo
Language:Inglês
Published: 1980
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC350199/
https://ncbi.nlm.nih.gov/pubmed/6255474
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