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Growth of T-lymphoma cells in serum-free medium: lack of involvement of the cyclic AMP pathway in long-term cultures.

We have developed a serum-free, chemically defined growth medium containing casein, insulin, transferrin, testosterone, and linoleic acid in Dulbecco's modified Eagle's medium/Ham's F12 medium, 1:1 (vol/vol), for growing murine T lymphomas. This medium supports the growth in suspensio...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Darfler, F J, Murakami, H, Insel, P A
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1980
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC350199/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6255474/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.77.10.5993
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