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Photooxidation of Amplex Red to resorufin: implications of exposing the Amplex Red assay to light

The Amplex Red assay, a fluorescent assay for the detection of H(2)O(2), relies on the reaction of H(2)O(2) and colorless, nonfluorescent Amplex Red with a 1:1 stoichiometry to form colored, fluorescent resorufin, catalyzed by horseradish peroxidase (HRP). We have found that resorufin is artifactual...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: Zhao, Baozhong, Summers, Fiona A., Mason, Ronald P.
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: 2012
Gaiak:
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC3501008/
https://ncbi.nlm.nih.gov/pubmed/22765927
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.freeradbiomed.2012.06.034
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