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Photooxidation of Amplex Red to resorufin: implications of exposing the Amplex Red assay to light
The Amplex Red assay, a fluorescent assay for the detection of H(2)O(2), relies on the reaction of H(2)O(2) and colorless, nonfluorescent Amplex Red with a 1:1 stoichiometry to form colored, fluorescent resorufin, catalyzed by horseradish peroxidase (HRP). We have found that resorufin is artifactual...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3501008/ https://ncbi.nlm.nih.gov/pubmed/22765927 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.freeradbiomed.2012.06.034 |
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