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Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
A simple and rapid method for transferring RNA from agarose gels to nitrocellulose paper for blot hybridization has been developed. Poly(A)+ and ribosomal RNAs transfer efficiently to nitrocellulose paper in high salt (3 M NaCl/0.3 M trisodium citrate) after denaturation with glyoxal and 50% (vol/vo...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Autor principal: | |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1980
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC350025/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6159641/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.77.9.5201 |
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