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Site-specific chemical protein conjugation using genetically encoded aldehyde tags
We describe a method for modifying proteins site-specifically using a chemoenzymatic bioconjugation approach. Formylglycine generating enzyme (FGE) recognizes a pentapeptide consensus sequence, CxPxR, and it specifically oxidizes the cysteine in this sequence to an unusual aldehyde-bearing formylgly...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3498491/ https://ncbi.nlm.nih.gov/pubmed/22576105 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2012.045 |
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