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Antibody production by single, hapten-specific B lymphocytes: an antigen-driven cloning system free of filler or accessory cells.
CBA mouse spleen cells were subjected to hapten affinity fractionation on thin layers of fluorescein (FLU)-gelatin. This procedure yields 97% B cells with varying FLU-binding avidities. One to 30 cells were placed in 10-microliter microcultures without any filler or accessory cells. the T-independen...
में बचाया:
| मुख्य लेखकों: | , , |
|---|---|
| स्वरूप: | Artigo |
| भाषा: | Inglês |
| प्रकाशित: |
1981
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| विषय: | |
| ऑनलाइन पहुंच: | https://ncbi.nlm.nih.gov/pmc/articles/PMC349338/ https://ncbi.nlm.nih.gov/pubmed/6174979 |
| टैग : |
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