Antibody production by single, hapten-specific B lymphocytes: an antigen-driven cloning system free of filler or accessory cells.

CBA mouse spleen cells were subjected to hapten affinity fractionation on thin layers of fluorescein (FLU)-gelatin. This procedure yields 97% B cells with varying FLU-binding avidities. One to 30 cells were placed in 10-microliter microcultures without any filler or accessory cells. the T-independen...

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Pubblicato in:	Proc Natl Acad Sci U S A
Autori principali:	Vaux, D L, Pike, B L, Nossal, G J
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	National Academy of Sciences 1981
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC349338/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6174979/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.78.12.7702
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