Antibody production by single, hapten-specific B lymphocytes: an antigen-driven cloning system free of filler or accessory cells.
CBA mouse spleen cells were subjected to hapten affinity fractionation on thin layers of fluorescein (FLU)-gelatin. This procedure yields 97% B cells with varying FLU-binding avidities. One to 30 cells were placed in 10-microliter microcultures without any filler or accessory cells. the T-independen...
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| Publicat a: | Proc Natl Acad Sci U S A |
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| Autors principals: | , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
National Academy of Sciences
1981
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC349338/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6174979/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.78.12.7702 |
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