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Antibody production by single, hapten-specific B lymphocytes: an antigen-driven cloning system free of filler or accessory cells.
CBA mouse spleen cells were subjected to hapten affinity fractionation on thin layers of fluorescein (FLU)-gelatin. This procedure yields 97% B cells with varying FLU-binding avidities. One to 30 cells were placed in 10-microliter microcultures without any filler or accessory cells. the T-independen...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1981
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC349338/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6174979/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.78.12.7702 |
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