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Antibody production by single, hapten-specific B lymphocytes: an antigen-driven cloning system free of filler or accessory cells.

CBA mouse spleen cells were subjected to hapten affinity fractionation on thin layers of fluorescein (FLU)-gelatin. This procedure yields 97% B cells with varying FLU-binding avidities. One to 30 cells were placed in 10-microliter microcultures without any filler or accessory cells. the T-independen...

Täydet tiedot

Tallennettuna:
Bibliografiset tiedot
Päätekijät: Vaux, D L, Pike, B L, Nossal, G J
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: 1981
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC349338/
https://ncbi.nlm.nih.gov/pubmed/6174979
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