A DNA fragment with an alpha-phosphorothioate nucleotide at one end is asymmetrically blocked from digestion by exonuclease III and can be replicated in vivo.

2'-Deoxyadenosine 5'-O-(1-thiotriphosphate) (dATP[alpha S]) was introduced into the 3' ends of DNA restriction fragments with Escherichia coli DNA polymerase I to give phosphorothioate internucleotide linkages. Such "capped" 3' ends were found to be resistant to exonuclease III digestion. Moreover,...

Volledige beschrijving

Bibliografische gegevens

Gepubliceerd in:	Proc Natl Acad Sci U S A
Hoofdauteurs:	Putney, S D, Benkovic, S J, Schimmel, P R
Formaat:	Artigo
Taal:	Inglês
Gepubliceerd in:	National Academy of Sciences 1981
Onderwerpen:	Research Article
Online toegang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC349264/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6278470/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.78.12.7350
Tags:	Voeg label toe Geen labels, Wees de eerste die dit record labelt!