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Design of pH Sensitive Binding Proteins from the Hyperthermophilic Sso7d Scaffold

We have engineered pH sensitive binding proteins for the Fc portion of human immunoglobulin G (hIgG) (hFc) using two different strategies – histidine scanning and random mutagenesis. We obtained an hFc-binding protein, Sso7d-hFc, through mutagenesis of the Sso7d protein from the hyperthermophilic ar...

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Autors principals: Gera, Nimish, Hill, Andrew B., White, Dalon P., Carbonell, Ruben G., Rao, Balaji M.
Format: Artigo
Idioma:Inglês
Publicat: Public Library of Science 2012
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3492137/
https://ncbi.nlm.nih.gov/pubmed/23145025
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0048928
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