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Rapid Fibroblast Removal from High Density Human Embryonic Stem Cell Cultures
Mouse embryonic fibroblasts (MEFs) were used to establish human embryonic stem cells (hESCs) cultures after blastocyst isolation(1). This feeder system maintains hESCs from undergoing spontaneous differentiation during cell expansion. However, this co-culture method is labor intensive, requires high...
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| Main Authors: | , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
MyJove Corporation
2012
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3490304/ https://ncbi.nlm.nih.gov/pubmed/23128236 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/3951 |
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