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Rapid Fibroblast Removal from High Density Human Embryonic Stem Cell Cultures

Mouse embryonic fibroblasts (MEFs) were used to establish human embryonic stem cells (hESCs) cultures after blastocyst isolation(1). This feeder system maintains hESCs from undergoing spontaneous differentiation during cell expansion. However, this co-culture method is labor intensive, requires high...

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Bibliografiske detaljer
Main Authors: Turner, William S., McCloskey, Kara E.
Format: Artigo
Sprog:Inglês
Udgivet: MyJove Corporation 2012
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3490304/
https://ncbi.nlm.nih.gov/pubmed/23128236
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/3951
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