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Adenovirus-mediated Genetic Removal of Signaling Molecules in Cultured Primary Mouse Embryonic Fibroblasts

The ability to genetically remove specific components of various cell signalling cascades has been an integral tool in modern signal transduction analysis. One particular method to achieve this conditional deletion is via the use of the Cre-loxP system. This method involves flanking the gene of inte...

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Detalhes bibliográficos
Main Authors: Hawley, Steve P., Wills, Melanie K. B., Jones, Nina
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3157869/
https://ncbi.nlm.nih.gov/pubmed/20864926
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/2160
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