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Physiological Induction of the Acrosome Reaction in Human Sperm: Validation of a Microassay Using Minimal Volumes of Solubilized, Homologous Zona Pellucida
Purpose: To develop a method that could accommodatemicrovolumes of solubilized human zona pellucida (ZP) andsperm for assessing the induction of the acrosome reaction. Methods: A microassay using 1 μl of 2.5, 1.25, 0.6, 0.3,and 0.125 ZP/μl incubated with 1 μl of a highly motilesperm suspension for 6...
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| Main Authors: | , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Kluwer Academic Publishers-Plenum Publishers
2000
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3489419/ https://ncbi.nlm.nih.gov/pubmed/11077617 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1023/A:1009493708268 |
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