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Physiology: Physiological Induction of the Acrosome Reaction in Human Sperm: Validation of a Microassay Using Minimal Volumes of Solubilized, Homologous Zona Pellucida
Purpose: The objective was to develop a method that couldaccommodate microvolumes of solubilized human (ZP) andsperm for assessing the induction of the acrosome reaction. Methods: A microassay using 1 μl of 2.5, 1.25, 0.6, 0.3,and 0.125 ZP/μl incubated with 1 μl of a highly motilesperm suspension fo...
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| Auteurs principaux: | , , |
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| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Kluwer Academic Publishers-Plenum Publishers
2000
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3455657/ https://ncbi.nlm.nih.gov/pubmed/10911576 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1023/A:1009418222397 |
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