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Electrotransformation of Clostridium thermocellum
Electrotransformation of several strains of Clostridium thermocellum was achieved using plasmid pIKm1 with selection based on resistance to erythromycin and lincomycin. A custom-built pulse generator was used to apply a square 10-ms pulse to an electrotransformation cuvette consisting of a modified...
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Main Authors: | , , |
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Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
American Society for Microbiology
2004
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC348934/ https://ncbi.nlm.nih.gov/pubmed/14766568 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.70.2.883-890.2004 |
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