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Electrotransformation of Clostridium thermocellum

Electrotransformation of several strains of Clostridium thermocellum was achieved using plasmid pIKm1 with selection based on resistance to erythromycin and lincomycin. A custom-built pulse generator was used to apply a square 10-ms pulse to an electrotransformation cuvette consisting of a modified...

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Detalhes bibliográficos
Main Authors: Tyurin, Michael V., Desai, Sunil G., Lynd, Lee R.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2004
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC348934/
https://ncbi.nlm.nih.gov/pubmed/14766568
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.70.2.883-890.2004
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