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Long-term culture of normal mouse B lymphocytes.

A procedure is described for the preparation of long-term lines of normal mouse B lymphocytes. Surface immunoglobulin-bearing splenic B lymphocytes were purified with the fluorescence-activated cell sorter and then cultured with lipopolysaccharide for 1-4 wk. The cells were then transferred into med...

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Detalhes bibliográficos
Main Authors: Howard, M, Kessler, S, Chused, T, Paul, W E
Formato: Artigo
Idioma:Inglês
Publicado em: 1981
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC348863/
https://ncbi.nlm.nih.gov/pubmed/6795635
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