Long-term culture of normal mouse B lymphocytes.
A procedure is described for the preparation of long-term lines of normal mouse B lymphocytes. Surface immunoglobulin-bearing splenic B lymphocytes were purified with the fluorescence-activated cell sorter and then cultured with lipopolysaccharide for 1-4 wk. The cells were then transferred into med...
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| Опубликовано в:: | Proc Natl Acad Sci U S A |
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| Главные авторы: | , , , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
National Academy of Sciences
1981
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC348863/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6795635/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.78.9.5788 |
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