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Directed evolution of the forkhead-associated domain to generate anti-phosphospecific reagents by phage-display
While affinity reagents are valuable tools for monitoring protein phosphorylation and studying signaling events in cells, generating them through immunization of animals with phosphopeptides is expensive, laborious and time consuming. An attractive alternative is to use protein evolution techniques...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3488158/ https://ncbi.nlm.nih.gov/pubmed/22985966 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2012.09.006 |
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