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A sensitive method for rapid detection of alkyl halides and dehalogenase activity using a multistep enzyme assay
A method for the detection of haloalkane conversion to the corresponding alcohols by haloalkane dehalogenases is described. It is based on a multistage enzyme reaction which allows for the analysis of alkyl halides in buffered systems. Irreversible hydrolytic dehalogenation catalyzed by haloalkane d...
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| Main Authors: | , , , , , |
|---|---|
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
Springer
2012
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3487978/ https://ncbi.nlm.nih.gov/pubmed/23006907 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/2191-0855-2-51 |
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