Enhanced cell disruption strategy in the release of recombinant hepatitis B surface antigen from Pichia pastoris using response surface methodology

BACKGROUND: Cell disruption strategies by high pressure homogenizer for the release of recombinant Hepatitis B surface antigen (HBsAg) from Pichia pastoris expression cells were optimized using response surface methodology (RSM) based on the central composite design (CCD). The factors studied includ...

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Detalhes bibliográficos
Main Authors: Tam, Yew Joon, Allaudin, Zeenathul Nazariah, Lila, Mohd Azmi Mohd, Bahaman, Abdul Rani, Tan, Joo Shun, Rezaei, Morvarid Akhavan
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2012
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3487952/
https://ncbi.nlm.nih.gov/pubmed/23039947
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-12-70
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