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Reversible Inhibition of Fusion Activity of a Paramyxovirus Fusion Protein by an Engineered Disulfide Bond in the Membrane-Proximal External Region

Cysteines were introduced into the membrane-proximal external region (MPER) of the paramyxovirus F protein. A disulfide bond formed, and the mutant protein was expressed at the cell surface but was fusion inactive. Reduction of the disulfide bond restored fusion activity. The data indicate that in a...

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Autori principali: Zokarkar, Aarohi, Connolly, Sarah A., Jardetzky, Theodore S., Lamb, Robert A.
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology 2012
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3486459/
https://ncbi.nlm.nih.gov/pubmed/22951841
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JVI.02006-12
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