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Chemical mechanisms for cytochrome P-450 hydroxylation: evidence for acylation of heme-bound dioxygen.

Using isotopic tracer methods, we have shown that dihydrolipoic acid (2,3-thioctic acid) acylates the distal oxygen of ferrous oxygenated Pseudomonas cytochrome P-450, forming a transient acyl peroxide intermediate that facilitates oxygen-oxygen bond cleavage. Single-turnover studies with 18O2 indic...

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Autors principals: Sligar, S G, Kennedy, K A, Pearson, D C
Format: Artigo
Idioma:Inglês
Publicat: 1980
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC348467/
https://ncbi.nlm.nih.gov/pubmed/6929480
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