Renaturation and localization of enzymes in polyacrylamide gels: Studies with UDPglucose pyrophosphorylase of Dictyostelium
We have developed procedures for renaturing, in polyacrylamide isoelectric focusing gels, homomeric enzymes (i.e., enzymes with identical subunits) that have been denatured with sodium dodecyl sulfate or urea or both. The renatured enzymes can then be localized as discrete species by conventional hi...
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| Publicat a: | Proc Natl Acad Sci U S A |
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| Autors principals: | , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
National Academy of Sciences
1980
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC348354/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6153803/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.77.2.730 |
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