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Renaturation and localization of enzymes in polyacrylamide gels: Studies with UDPglucose pyrophosphorylase of Dictyostelium

We have developed procedures for renaturing, in polyacrylamide isoelectric focusing gels, homomeric enzymes (i.e., enzymes with identical subunits) that have been denatured with sodium dodecyl sulfate or urea or both. The renatured enzymes can then be localized as discrete species by conventional hi...

Whakaahuatanga katoa

I tiakina i:
Ngā taipitopito rārangi puna kōrero
I whakaputaina i:Proc Natl Acad Sci U S A
Ngā kaituhi matua: Manrow, Richard E., Dottin, Robert P.
Hōputu: Artigo
Reo:Inglês
I whakaputaina: National Academy of Sciences 1980
Ngā marau:
Urunga tuihono:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC348354/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6153803/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.77.2.730
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