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Delipidation of bacteriorhodopsin and reconstitution with exogenous phospholipid.
Solubilizations of the purple membrane from Halobacterium halobium with the detergent Tritain X-100 followed by gel filtration in deoxycholate solution gave bacteriorhodopsin that was more than 99% free from endogenous lipid. The delipidated bacteriorhodopsin was reconstituted with exogenous phospho...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1980
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC348262/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6928624/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.77.1.323 |
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