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Delipidation of bacteriorhodopsin and reconstitution with exogenous phospholipid.

Solubilizations of the purple membrane from Halobacterium halobium with the detergent Tritain X-100 followed by gel filtration in deoxycholate solution gave bacteriorhodopsin that was more than 99% free from endogenous lipid. The delipidated bacteriorhodopsin was reconstituted with exogenous phospho...

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Detalhes bibliográficos
Main Authors: Huang, K S, Bayley, H, Khorana, H G
Formato: Artigo
Idioma:Inglês
Publicado em: 1980
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC348262/
https://ncbi.nlm.nih.gov/pubmed/6928624
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