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Counting protein molecules using quantitative fluorescence microscopy

In recent years, quantification of absolute protein numbers in cellular structures using fluorescence microscopy has become a reality. Two popular methods are available to a broad range of researchers with minimal equipment and analysis requirements: stepwise photobleaching to count discrete changes...

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Bibliographic Details
Main Authors: Coffman, Valerie C., Wu, Jian-Qiu
Format: Artigo
Language:Inglês
Published: 2012
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3482307/
https://ncbi.nlm.nih.gov/pubmed/22948030
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.tibs.2012.08.002
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