An Optimized Method for Computing (18)O/(16)O Ratios of Differentially Stable-isotope Labeled Peptides in the Context of Post-digestion (18)O Exchange/Labeling

Differential (18)O/(16)O stable isotope labeling of peptides that relies on enzyme-catalyzed oxygen exchange at their carboxyl termini in the presence of H(2)(18)O has been widely used for relative quantitation of peptides/proteins. The role of tryptic proteolysis in bottom-up shotgun proteomics and...

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Hlavní autoři: Ye, Xiaoying, Luke, Brian T., Johann, Donald J., Ono, Akira, Chan, King C., Prieto, DaRue A., Issaq, Haleem J., Veenstra, Timothy D., Blonder, Josip
Médium: Artigo
Jazyk:Inglês
Vydáno: 2010
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On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3479679/
https://ncbi.nlm.nih.gov/pubmed/20540505
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac101284c
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