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An Optimized Method for Computing (18)O/(16)O Ratios of Differentially Stable-isotope Labeled Peptides in the Context of Post-digestion (18)O Exchange/Labeling
Differential (18)O/(16)O stable isotope labeling of peptides that relies on enzyme-catalyzed oxygen exchange at their carboxyl termini in the presence of H(2)(18)O has been widely used for relative quantitation of peptides/proteins. The role of tryptic proteolysis in bottom-up shotgun proteomics and...
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| Main Authors: | , , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3479679/ https://ncbi.nlm.nih.gov/pubmed/20540505 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac101284c |
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