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Quantitative mass spectrometry and PAR-CLIP to identify RNA-protein interactions

Systematic analysis of the RNA-protein interactome requires robust and scalable methods. We here show the combination of two completely orthogonal, generic techniques to identify RNA-protein interactions: PAR-CLIP reveals a collection of RNAs bound to a protein whereas SILAC-based RNA pull-downs ide...

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Bibliografische gegevens
Hoofdauteurs: Scheibe, Marion, Butter, Falk, Hafner, Markus, Tuschl, Thomas, Mann, Matthias
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Oxford University Press 2012
Onderwerpen:
RNA
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3479200/
https://ncbi.nlm.nih.gov/pubmed/22885304
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gks746
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