Nanoflow electrospinning serial femtosecond crystallography

An electrospun liquid microjet has been developed that delivers protein microcrystal suspensions at flow rates of 0.14–3.1 µl min(−1) to perform serial femtosecond crystallography (SFX) studies with X-ray lasers. Thermolysin microcrystals flowed at 0.17 µl min(−1) and diffracted to beyond 4 Å resolu...

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Hauptverfasser: Sierra, Raymond G., Laksmono, Hartawan, Kern, Jan, Tran, Rosalie, Hattne, Johan, Alonso-Mori, Roberto, Lassalle-Kaiser, Benedikt, Glöckner, Carina, Hellmich, Julia, Schafer, Donald W., Echols, Nathaniel, Gildea, Richard J., Grosse-Kunstleve, Ralf W., Sellberg, Jonas, McQueen, Trevor A., Fry, Alan R., Messerschmidt, Marc M., Miahnahri, Alan, Seibert, M. Marvin, Hampton, Christina Y., Starodub, Dmitri, Loh, N. Duane, Sokaras, Dimosthenis, Weng, Tsu-Chien, Zwart, Petrus H., Glatzel, Pieter, Milathianaki, Despina, White, William E., Adams, Paul D., Williams, Garth J., Boutet, Sébastien, Zouni, Athina, Messinger, Johannes, Sauter, Nicholas K., Bergmann, Uwe, Yano, Junko, Yachandra, Vittal K., Bogan, Michael J.
Format: Artigo
Sprache:Inglês
Veröffentlicht: International Union of Crystallography 2012
Schlagworte:
Short Communications
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3478121/
https://ncbi.nlm.nih.gov/pubmed/23090408
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S0907444912038152
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