Exploring optimization parameters to increase ssDNA recombineering in Lactococcus lactis and Lactobacillus reuteri

Single-stranded DNA (ssDNA) recombineering is a technology which is used to make subtle changes in the chromosome of several bacterial genera. Cells which express a single-stranded DNA binding protein (RecT or Bet) are transformed with an oligonucleotide which is incorporated via an annealing and re...

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Main Authors: van Pijkeren, Jan-Peter, Neoh, Kar Mun, Sirias, Denise, Findley, Anthony S., Britton, Robert A.
Format: Artigo
Sprog:Inglês
Udgivet: Landes Bioscience 2012
Fag:
Research Paper
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3476877/
https://ncbi.nlm.nih.gov/pubmed/22750793
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4161/bioe.21049
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