A Faster, High Resolution, mtPA-GFP-based Mitochondrial Fusion Assay Acquiring Kinetic Data of Multiple Cells in Parallel Using Confocal Microscopy

Mitochondrial fusion plays an essential role in mitochondrial calcium homeostasis, bioenergetics, autophagy and quality control. Fusion is quantified in living cells by photo-conversion of matrix targeted photoactivatable GFP (mtPAGFP) in a subset of mitochondria. The rate at which the photoconverte...

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Bibliographic Details
Main Authors: Lovy, Alenka, Molina, Anthony J.A., Cerqueira, Fernanda M., Trudeau, Kyle, Shirihai, Orian S.
Format: Artigo
Language:Inglês
Published: MyJove Corporation 2012
Subjects:
Molecular Biology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3476419/
https://ncbi.nlm.nih.gov/pubmed/22847388
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/3991
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