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Replication of lambda dv plasmid in vitro promoted by purified lambda O and P proteins.

An in vitro system for replication of lambda dv plasmid DNA has been constructed. This system consists of an ammonium sulfate fraction from Escherichia coli extract, exogenously added purified lambda O and P proteins, and lambda dv DNA in closed circular form. More than 85% of the added template DNA...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Tsurimoto, T, Matsubara, K
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1982
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC347403/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6218500/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.79.24.7639
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