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Replication of lambda dv plasmid in vitro promoted by purified lambda O and P proteins.

An in vitro system for replication of lambda dv plasmid DNA has been constructed. This system consists of an ammonium sulfate fraction from Escherichia coli extract, exogenously added purified lambda O and P proteins, and lambda dv DNA in closed circular form. More than 85% of the added template DNA...

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Detalhes bibliográficos
Main Authors: Tsurimoto, T, Matsubara, K
Formato: Artigo
Idioma:Inglês
Publicado em: 1982
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC347403/
https://ncbi.nlm.nih.gov/pubmed/6218500
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