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Local mutagenesis within deletion loops of DNA heteroduplexes.

An efficient method has been developed to generate base substitution mutations within deletion loops of DNA heteroduplexes. This method utilizes a heteroduplex formed between a deletion mutant cloned in a plasmid vector and its wild-type counterpart from which two restriction sites had been removed...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Peden, K W, Nathans, D
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1982
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC347309/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6760191/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.79.23.7214
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