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Site Directed Mutagenesis of Schizosaccharomyces pombe Glutathione Synthetase Produces an Enzyme with Homoglutathione Synthetase Activity

Three different His-tagged, mutant forms of the fission yeast glutathione synthetase (GSH2) were derived by site-directed mutagenesis. The mutant and wild-type enzymes were expressed in E. coli DH5α and affinity purified in a two-step procedure. Analysis of enzyme activity showed that it was possibl...

وصف كامل

محفوظ في:
التفاصيل البيبلوغرافية
المؤلفون الرئيسيون: Dworeck, Tamara, Zimmermann, Martin
التنسيق: Artigo
اللغة:Inglês
منشور في: Public Library of Science 2012
الموضوعات:
الوصول للمادة أونلاين:https://ncbi.nlm.nih.gov/pmc/articles/PMC3473041/
https://ncbi.nlm.nih.gov/pubmed/23091597
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0046580
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