Thiol-beta-lactamase: replacement of the active-site serine of RTEM beta-lactamase by a cysteine residue.
We describe a procedure by which the codon (AGC) for the active-site serine-70 of pBR322 beta-lactamase (penicillinase, penicillin amido-beta-lactamhydrolase, EC 3.5.2.6) is altered to that for cysteine (TGC). The pertinent nucleotide bases, A-G-C-A, positions 410-413, of pBR322 are excised by treat...
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| Publicat a: | Proc Natl Acad Sci U S A |
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| Autors principals: | , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
National Academy of Sciences
1982
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC347297/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6818541/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.79.23.7157 |
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