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Thiol-beta-lactamase: replacement of the active-site serine of RTEM beta-lactamase by a cysteine residue.

We describe a procedure by which the codon (AGC) for the active-site serine-70 of pBR322 beta-lactamase (penicillinase, penicillin amido-beta-lactamhydrolase, EC 3.5.2.6) is altered to that for cysteine (TGC). The pertinent nucleotide bases, A-G-C-A, positions 410-413, of pBR322 are excised by treat...

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Detalhes bibliográficos
Main Authors: Sigal, I S, Harwood, B G, Arentzen, R
Formato: Artigo
Idioma:Inglês
Publicado em: 1982
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC347297/
https://ncbi.nlm.nih.gov/pubmed/6818541
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