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Combination of Oxyanion Gln114 Mutation and Medium Engineering to Influence the Enantioselectivity of Thermophilic Lipase from Geobacillus zalihae

The substitution of the oxyanion Q114 with Met and Leu was carried out to investigate the role of Q114 in imparting enantioselectivity on T1 lipase. The mutation improved enantioselectivity in Q114M over the wild-type, while enantioselectivity in Q114L was reduced. The enantioselectivity of the ther...

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Detalhes bibliográficos
Main Authors: Wahab, Roswanira Abdul, Basri, Mahiran, Rahman, Mohd Basyaruddin Abdul, Rahman, Raja Noor Zaliha Raja Abdul, Salleh, Abu Bakar, Leow, Thean Chor
Formato: Artigo
Idioma:Inglês
Publicado em: Molecular Diversity Preservation International (MDPI) 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3472768/
https://ncbi.nlm.nih.gov/pubmed/23109876
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/ijms130911666
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