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Cloning and sequence determination of the gene for the human immunoglobulin epsilon chain expressed in a myeloma cell line.

Messenger RNA has been isolated from cells of the human myeloma line 266BL which synthesizes IgE of the myeloma ND. A fraction enriched in mRNA for the epsilon heavy chain was copied into DNA and the DNA was cloned in Escherichia coli. A chemically synthesized oligonucleotide probe, based on the exp...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:Proc Natl Acad Sci U S A
Main Authors: Kenten, J H, Molgaard, H V, Houghton, M, Derbyshire, R B, Viney, J, Bell, L O, Gould, H J
Format: Artigo
Jezik:Inglês
Izdano: National Academy of Sciences 1982
Teme:
Online dostop:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC347188/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6815656/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.79.21.6661
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